Does it matter what time of the day I give the protocol supplements? Does giving it earlier in the day or later at night have an impact on anything?

The best way to administer this protocol daily is the one that suits you best and makes your life most manageable. You can give all the supplements together simultaneously or provide them at different times of the day. You can split them up into two smaller doses or give them a full dose once a day. You can administer it at night or in the morning. Consistency is key to success, and working this into your routine and making it a habit is the best way to ensure that you will adhere to it long term. Giving some aspects of the protocol in the morning vs. in the evening does not make any discernible difference so stick to what works for your family.